›› 2012, Vol. 7 ›› Issue (6): 260-267.doi:

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Evaluation of antioxidant and anticancer potential of Cassia tora leaves

  

  • 收稿日期:2012-09-29 修回日期:2013-03-18 出版日期:2012-12-20 发布日期:2012-12-20

Evaluation of antioxidant and anticancer potential of Cassia tora leaves

Jinu John 1*, Archana Mehta 2, Pradeep Mehta 2
  

  1. 1 Inter University Instrumentation Centre, Mahatma Gandhi University, Kottayam, Kerala, India; 2 School of Chemical and Biological Sciences, Dr. Hari Singh Gour University, Sagar, Madhya Pradesh, India
  • Received:2012-09-29 Revised:2013-03-18 Online:2012-12-20 Published:2012-12-20
  • Contact: Jinu John

Abstract: Cassia tora Linn. belongs to Leguminosae family has been traditionally reported to have medicinal properties. Reports on phyto-pharmacological evaluation of C. tora leaves for antioxidant and anticancer potential are rare. The aim of this study is to evaluate the anticancer potential, antioxidant activity and the total phenolic contents of C. tora leaf extracts. Methenolic extract and its ethyl acetate fraction were used for the analysis. Antioxidant assay was performed by in vitro models such as 2, 2-Diphenyl-1-picrylhydrazyl (DPPH), nitric oxide radical (NO.) scavenging and total reducing activity. The results were compared with that of standard compounds. Total phenolic content was measured by Folin-Ciocalteu’s reagent method using gallic acid as standard. Cytotoxicity of extracts was analyzed by sulforhodamine B (SRB) assay on cancer cell lines (MCF -7, SiHa, SK.N.SH, IMR-32, HT-29 and OVCAR-5). Ethyl acetate fraction showed highest percentage of phenolics (32.439%), relatively better antioxidant and cytotoxic property. Our results indicate that phenolic content may be responsible for the antioxidant activity of plant extracts and the observations were supportive to the traditional use of the plant as a nutraceutical leafy vegetable.

Key words: Cassia tora leaves, antioxidant potential, phenolic content, anticancer potential