亚洲传统医药

Abstract: Scopoletin and mangiferin are two phenolic compounds detected in Canscora decussata (Gentianaceae). A simple, accurate and sensitive method for their determination in crude drug was developed using spectrofluorimetry. Scopoletin exhibited a strong bright blue fluorescence at excitation and emission wavelengths of 430 nm and 460 nm, respectively, and mangiferin exhibited a strong apricot yellow green fluorescence at excitation and emission wavelengths of 248 nm and 520 nm, respectively. The method was statistically validated and found suitable for quantitation of mangiferin and scopoletin in the methanolic extract of C. decussata. The proposed spectrofluorimetric method provides a faster and low cost quality control by simultaneous routine analysis of scopoletin and mangiferin in C. decussata and its formulations.

Key words: Canscora decussata, gentianaceae, spectrofluorimetry, coumarin, xanthone, scopoletin, mangiferin, shankhpushpi