亚洲传统医药
亚洲传统医药

快速检索 年期检索

›› 2010, Vol. 5 ›› Issue (3): 79-88.

• Regular Articles •    下一篇

In vitro hepatoprotective and antioxidant activities of the crude extract and isolated compounds from Irvingia gabonensis

  

  • 收稿日期:2009-07-21 修回日期:2010-05-08 出版日期:2010-06-20 发布日期:2010-06-20

In vitro hepatoprotective and antioxidant activities of the crude extract and isolated compounds from Irvingia gabonensis

J. Hubert Donfack 1 *; G. Wabo Fotso 2; B. Ngameni 3; F. Ngueguim Tsofack 4 ; A. Tchoukoua 2; P. Ambassa 2; W. Abia 1; A. N. Tchana 1; S. Giardina6; D. Buonocore 6; P. Vita Finzi 5; G. Vidari 5;
F. Marzatico 6; B T. Ngadjui 2; P. F. Moundipa 1
  

  1. 1 Laboratory of Pharmacology and Toxicology, Department of Biochemistry, Faculty of Science, University of Yaounde I, P.O. Box 812 Yaounde, Cameroon; 2 Laboratory of Organic Chemistry, Department of Organic Chemistry, Faculty of Science, University of Yaounde I, P.O. Box 812 Yaounde, Cameroon; 3 Department of Pharmacy and Traditional Pharmacotherapeutics, Faculty of Medicine and Biomedical Science, University of Yaoundé I, B.P. 1364 Yaoundé, Cameroon; 4 Laboratory of Animal Physiology, Department of Animal Biology and Physiology, Faculty of Science, University of Yaounde I, P.O. Box 812 Yaounde, Cameroon; 5 Dipartimento di Chimica Organica, Università degli Studi di Pavia and Centre of Ethnobiopharmacy (CISTRE), Via Taramelli 10, 27100 Pavia, Italy; 6 Dipartimento di Scienze Fisiologiche-Farmacologiche Cellulari e Molecolari. Sezione di Farmacologia e Biotecnologie Farmacologiche, Piazza Botta 11-27100, Italy
  • Received:2009-07-21 Revised:2010-05-08 Online:2010-06-20 Published:2010-06-20
  • Contact: J. Hubert Donfack

Abstract: The in vitro hepatoprotective effect of the methanolic extract from Irvingia gabonensis (Aubry Lecomte ex O’Rorke) on CCl4-induced liver cell damage as well as the possible antioxidant mechanisms involved in this protective effect were investigated. The phytochemical investigation of this methanolic extract led to the isolation of seven compounds identified as: 3-friedelanone (1); betulinic acid (2); oleanolic acid (3); 3, 3’, 4’-tri-O-trimethylellagic acid (4); methyl gallate (5); hardwickiic acid (6) and 3-β-acetoxyursolic acid (7). The hepatoprotective activity of these compounds was tested in vitro against CCl4-induced damage in rat hepatoma cells. Compounds (3), (7), (5) and (2) showed significant hepatoprotective activity as indicated by their ability to prevent liver cell death and LDH leakage during CCl4 intoxication. In addition, 2, 4-dinitrophenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, the β-Carotene-Linoleic Acid Model System (β-CLAMS), the Ferric-Reducing Antioxidant Parameter (FRAP) and microsomal lipid peroxidation assays were used to measure the antioxidant activity of the crude extract and isolated compounds. Compared with compounds (1), (2), (4), and (6), compounds (3), (5) and (7) showed significant antioxidant effects involving radical-scavenging action, inhibition of microsomal lipid peroxidation, β-CLAMS and FRAP assays. These results suggest that the protective effects of the crude extract of Irvingia gabonensis against CCl4-induced hepatotoxicity possibly involve the antioxidant effect of these compounds.

Key words: Irvingia gabonensis, hepatoprotective effects, antioxidant activity, carbon tetrachloride, plant compounds

版权所有 © 2019 《亚洲传统医药》

本系统由北京玛格泰克科技发展有限公司设计开发