亚洲传统医药
亚洲传统医药

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›› 2015, Vol. 10 ›› Issue (2): 46-56.

• 论文 • 上一篇    

Highly Effectively Evaluating the quality consistency ofDihuang Pills by Quantified both HPLC and IR profilesthat assisted by antioxidant activity

Xinxin Chen 1, Guoxiang Sun 1*, Jiayue Gao 1, Lei Wang 1, Yue Zou 1   

  1. 1 Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning 110016, China
  • 收稿日期:2014-12-20 修回日期:2015-03-31 出版日期:2015-04-20 发布日期:2015-04-20

Highly Effectively Evaluating the quality consistency ofDihuang Pills by Quantified both HPLC and IR profilesthat assisted by antioxidant activity

Xinxin Chen 1, Guoxiang Sun 1*, Jiayue Gao 1, Lei Wang 1, Yue Zou 1   

  1. 1 Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning 110016, China
  • Received:2014-12-20 Revised:2015-03-31 Online:2015-04-20 Published:2015-04-20
  • Contact: Guoxiang Sun

摘要： A high performance liquid chromatography (HPLC) method was developed for fingerprint profiling to evaluate the quality
consistency of DHPs. Method validation results demonstrate that desired precision, repeatability, and a sample stability test was
achieved. The HPLC fingerprints of samples were evaluated by PCA and the similarity analysis introduced by us. The infrared
spectral fingerprint (IR-FP) was also achieved as a supplement of qualitative information. At last, DPPH assay was performed
to assess the antioxidant activity of DHPs, in vitro, which was correlated to the fingerprint components. This study demonstrates
that HPLC fingerprint combined with IR-FP and antioxidant ability provides a reliable and efficient methodology for the
assessment of quality of the TCM and herbal preparations.

Abstract: A high performance liquid chromatography (HPLC) method was developed for fingerprint profiling to evaluate the quality
consistency of DHPs. Method validation results demonstrate that desired precision, repeatability, and a sample stability test was
achieved. The HPLC fingerprints of samples were evaluated by PCA and the similarity analysis introduced by us. The infrared
spectral fingerprint (IR-FP) was also achieved as a supplement of qualitative information. At last, DPPH assay was performed
to assess the antioxidant activity of DHPs, in vitro, which was correlated to the fingerprint components. This study demonstrates
that HPLC fingerprint combined with IR-FP and antioxidant ability provides a reliable and efficient methodology for the
assessment of quality of the TCM and herbal preparations.

Key words: Liuwei Dihuang pill, HPLC fingerprint, IR-FP, antioxidant activity, quality control

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