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A systematic quantitative method for quality evaluation of Bupleurum chinense DC. based on multi-wavelength fusion fingerprint combined with multivariate chemometrics

Ye Zhu, Ran Wen, Yaping Yu, Wei Wei, Guoxiang Sun*   

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning, China
  • 收稿日期:2018-09-29 修回日期:2018-12-01 出版日期:2019-04-20 发布日期:2018-12-01

A systematic quantitative method for quality evaluation of Bupleurum chinense DC. based on multi-wavelength fusion fingerprint combined with multivariate chemometrics

Ye Zhu, Ran Wen, Yaping Yu, Wei Wei, Guoxiang Sun*   

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, Liaoning, China
  • Received:2018-09-29 Revised:2018-12-01 Online:2019-04-20 Published:2018-12-01
  • Contact: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; Tel.: +86-24-23986286; E-mail: gxswmwys@163. com.

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摘要： A quantitative evaluation method for the quality consistency of Bupleurum chinense DC. was developed by combining multiwavelength fusion fingerprint with chemometric methods. The fingerprint information of Bupleurum at five wavelengths (210, 254, 265, 280 and 320 nm) collected by HPLC-DAD instrument was fused to overall control its quality. The fusion fingerprints and four components (saikosaponin a, saikosaponin d, rutin, quercitrin) were studied by systematic quantitative fingerprint method and principal component analysis, respectively. Besides, fingerprint-efficacy relationship was constructed in vitro using partial least-squares model. In conclusion, all work can serve as a valid reference for the quality control of Bupleurum.

关键词: chemometrics, fingerprint-efficacy relationship, fusion fingerprint, Bupleurum chinense DC., quality consistency

Abstract: A quantitative evaluation method for the quality consistency of Bupleurum chinense DC. was developed by combining multiwavelength fusion fingerprint with chemometric methods. The fingerprint information of Bupleurum at five wavelengths (210, 254, 265, 280 and 320 nm) collected by HPLC-DAD instrument was fused to overall control its quality. The fusion fingerprints and four components (saikosaponin a, saikosaponin d, rutin, quercitrin) were studied by systematic quantitative fingerprint method and principal component analysis, respectively. Besides, fingerprint-efficacy relationship was constructed in vitro using partial least-squares model. In conclusion, all work can serve as a valid reference for the quality control of Bupleurum.

Key words: chemometrics, fingerprint-efficacy relationship, fusion fingerprint, Bupleurum chinense DC., quality consistency

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