亚洲传统医药

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2016年, 第11卷, 第1期　刊出日期：2016-02-20 上一期    下一期

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27 Fingerprint analysis of Clerodendranthus Spicatus from different regions by high-performance liquid chromatography 2016 (1):  1-10.  摘要 ( 280 )   PDF(970KB) ( 275 )   AIM: To develope a novel, simple and accurate fingerprint method for the quality control of Clerodendranthus Spicatus from different regions by high-performance liquid chromatography, which is widely used for urolithiasis. METHODS: Chromatographic separation was performed on Agilent SB-C18 (250 mm×4.6 mm, 5 μm) by the gradient elution of 0.1% H3PO4 in water and acetonitrile at 0.8 mL·min-1 at 210 nm by DAD detector. The column temperature was set at 30 °C. RESULTS: The method has good stability and reproducibility with RSD less than 2%. 10 peaks were selected as the common peaks. Rosmarinic acid was taken as a reference. The similarity of 10 batches was above 0.90. CONCLUSION: This method is simple and specific, which provided an effective way to assess the quality of Clerodendranthus Spicatus. 相关文章 | 计量指标

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Simultaneous Determination of genistein, genistin, daidzein, daidzin, glycitein and glycitin in different fermented products of Semen sojae praeparatum. 2016 (1):  11-18.  摘要 ( 257 )   PDF(969KB) ( 355 )   A simple and reliable ultra performance liquid chromatography method was established for determination of genistein, genistin, daidzein, daidzin, glycitein and glycitin content in different period fermentation of Semen sojae praeparatum. The chromatographic separation was performed on an ACQUITY UPLC BEH C18 (2.1*50 mm, 1.7 μm). The mobile phase was composed of acetonitrile (A), 0.1% formic acid in water (B). The gradient elution conditions were as follows: 0-8 min, 10-24% (A), 8-10 min, 24-50% (A), 10-12 min, 50% (A). The chromatogram was monitored at wavelength of 260 nm during the experiment. The result showed that the content of daidzin, glycitin and genistin were reduced, however the content of daidzein, glycitein and genistein were increased. The method is simple, accurate and reproducible. It can be used to provide the theory basis for the quality control of traditional Chinese medicine Semen sojae praeparatum. Finally, it provides an identification standard for production and market circulation of Semen sojae praeparatum. 相关文章 | 计量指标

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Pharmacokinetic studies on Schisandrae Fructus lignans and the affecting factors on pharmacokinetic actions of the lignans 2016 (1):  19-26.  摘要 ( 174 )   PDF(817KB) ( 273 )   The main lignans of Schisandra chinensis are known as the therapeutic components. The absorption, distribution, metabolites, and metabolic pathways of them were concluded in this paper. Potential effects on experiment results of different subjects were also discussed. Results: 1. Deoxyschisandrin (DS), schisandrin (SCH) and schisandrin B (SCH B/γ-SCH) can be regarded as the main therapeutic components absorbed into blood. 2. All the lignans showed the same distribution trend: highest concentration in liver and lungs, then in kidney and heart, least in spleen and intestines. DS and SCH can cross the blood-brain barrier (BBB). 3. CYP3A4 is the only subtype that catalysis metabolic pathway of DS and SCH. The metabolic order of DS and SCH differed in in vivo and in vitro environment. 4. After oral administration, the Tmax of DS was about 4~5 h, the Tmax of SCH distributed from 0.33 to 0.7 h. The secondary absorption of γ-SCH had been observed. 5. Gender difference is found in the metabolism of SCH and γ-SCH in vitro using the liver microsomes of male and female rats. The blood pretreatment methods had also been compared and using plasma turned to be a better choice than using serum for Schisandrae lignan analyzation. 相关文章 | 计量指标