Asian Journal
Of Traditional Medicines

Abstract: Duzhong Yaozhui Pill (DYP) as an orthopaedic medicine, it was evaluated in experimental models of inflammation, analgesic and toxicity. Xylene-induced ear edema in mice was used to assess anti-inflammation activity and analgesic was determined by acetic acid induced writhing in mice. Compared with control group, DYP exhibited significantly (p<0.05) anti-inflammatory effect through inhibiting ear edema caused by xylene with dose dependent manner. Compared with control group, DYP at the dosages of 2.36 mg/kg, 4.72 mg/kg showed significantly (p<0.05) analgesic activity by decreasing the mice writhing induced by acetic acid with dose dependent manner. The positive drug (Wantong Jingu Table) also presented anti-inflammatory and analgesic activity. In toxicity assays, male and female mice were given by gavage at the maximum dosage of DYP and observed indicators including clinical signs, animal mortality and the change of animal body weight. It was found that DYP was safe at dose of 42 g/kg and no mortality was shown even after 14 days of DYP administration. And the mice weight was gradually increased. This study gives support to the application of DYP in clinical.

Key words: Duzhong Yaozhui Pill, anti-inflammatory activity, analgesic activity, acute toxicity