Asian Journal
Of Traditional Medicines

Abstract: HPLC coupled with diode array detector (DAD) was adopted to monitor the quality consistency of Compound Liangmianzhen tablets (LMZT). An overall integrated fingerprint assessment strategy was set up to reveal the whole information at different wavelengths, and 220 nm HPLC fingerprints as well as single maker component were determined, in combination of antioxidant activity detected by 1,1-diphenyl-2-picrylhydrazyl (DPPH). The systematic quantitative fingerprint method (SQFM) was applied in the fingerprint assessments and we did mathematical separation for overlapping peaks which can’t be separated under chromatographic conditions before evaluation. The samples were all quantified and 11 kinds of components had antioxidant activity.

Key words: antioxidant activity, Compound Liangmianzhen tablets, HPLC fingerprints, partial least squares regression, systematic quantitative fingerprint method