›› 2006, Vol. 1 ›› Issue (3-4): 142-149.

• 论文 • Previous Articles     Next Articles

A Novel Approach to the Digitized Fingerprint of Radix Bupleuriby HPLC

Guoxiang Sun* ; Jindan Liu; Yumeng Bi; Kaishun Bi; Yuqing Sun   

  1. School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, China
  • Received:2006-01-01 Revised:2006-01-01 Online:2006-10-15 Published:2006-10-15
  • Contact: Guoxiang Sun

Abstract: High performance liquid chromatography was applied to the determination of the fingerprints of Radix Bupleuri. All the samples were analyzed by using a gradient elution with 1 % acetic acid in water/acetonitrile and with 1% acetic acid ( from 0 /100 to 50 /50, v /v, in 70 min ) as the mobile phase, a CenturySIL C18 AQ column ( 20 cm × 4.6 mm, 5 μm ) with the temperature of 30 ± 0.15 °C and a detection wavelength of 265 nm. The HPLC profiles produced from chromatograms of ten different batches of samples were established as characteristic fingerprints having 27 common peaks. The ten chromatograms were assessed by the software of the Digitized Evaluation System for Superinformation Characteristics of theTraditional Chinese Medicine Fingerprints chiefly in terms of similarities of the chemical constituent distribution and content. And the results showed a good similarity between the sample and the referential HPLC fingerprints. This method can exploit the potential information hidden in the HPLC fingerprints with high precision and reproducibility and serve as a perfect way of the quality control of Radix Bupleuri.

Key words: Radix Bupleuri, HPLC, digitized fingerprints, super information characteristics, content similarity P %, quantitative similarity C % calculated by the vector projection.

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