HPLC determination of two bioactive flavone glycosides and GC-MS analysis of volatile oil constituents in Alpinia nigra

›› 2007, Vol. 2 ›› Issue (3): 85-91.

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HPLC determination of two bioactive flavone glycosides and GC-MS analysis of volatile oil constituents in Alpinia nigra

Chunfeng Qiao ¹; Quanbin Han ¹; Jingzheng Song ¹; Zhengtao Wang ² *; Luoshan Xu ²; Hongxi Xu ¹ *

1 Chinese Medicine Laboratory, Hong Kong Jockey Club Institute of Chinese Medicine, Hong Kong, China; 2 Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210009, China.

Received:2007-01-01 Revised:2007-01-01 Online:2007-06-15 Published:2007-06-15
Contact: Hongxi Xu, Zhengtao Wang

Abstract

Abstract: Two bioactive flavone glycosides, astragalin and kaempferol-3-O-glucuronide, were analyzed in Alpinia nigra (Gaertn.) Burtt by HPLC. The method was validated in detail. Kaempferol-3-O-glucuronide was found to be a dominant compound in the seed clusters. It is primarily distributed in the pulp. In addition, volatile oils of fruits and rhizomes of A. nigra were studied by GC-MS. β-Pinene and α-pinene were found to be major constituents of the volatile oil of the fruits.

Key words: Alpinia nigra, astragalin, kaempferol-3-O-glucuronide, volatile oil, HPLC, GC-MS

Chunfeng Qiao;Quanbin Han;Jingzheng Song;Zhengtao Wang *;Luoshan Xu; Hongxi Xu * . HPLC determination of two bioactive flavone glycosides and GC-MS analysis of volatile oil constituents in Alpinia nigra [J]. , 2007, 2(3): 85-91.

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HPLC determination of two bioactive flavone glycosides and GC-MS analysis of volatile oil constituents in Alpinia nigra

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Asian Journal
Of Traditional Medicines