亚洲传统医药

Abstract: The 40 % hydroethanolic stem bark extract (HE40) from Erythrina senegalensis was subjected to purification by repeated chromatographies. Three diprenylated isoflavonoids were isolated and identified as 2, 3-dihydro-2’-hydroxyosajin (1), osajin (2) and 6, 8-diprenylgenistein (3). These compounds were tested for hepatoprotective activities against in vitro CCl4-induced hepatitis in rat liver slices. Four model systems: 2, 4-dinitrophenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, β-Carotene-Linoleic Acid Model System (β-CLAMS), Ferric-Reducing Antioxidant Power (FRAP) assay and microsomal lipid peroxydation were used to measure the antioxidant activity of these three isoflavones. The obtained hepatoprotective percentages of compounds (1) (2) (3) were 71.8 ± 1.45, 67.54 ± 3.56 and 69.41 ± 2.56 respectively. Compared to compound (2) and (3), compound (1) showed significant antioxidant effect with EC50 values of 41.28 ± 1.20, 31.73 ± 0.49, 19.17 ± 3.5 and 15.99 ± 3.10 µg/ml respectively for the radical-scavenging action, inhibition of microsomal lipid peroxydation, β-CLAMS and FRAP assays. The hepatoprotective activity of silymarin used as reference compound was lower than the activities of isolated compounds. The results obtained provide promising baseline information for the potential use of this crude extract as well as some of the isolated compounds for their hepatoprotective and antioxidant activities. It is also worth noting that these results validate with in vitro tests, the therapeutic use of the plant in traditional medicine.

Key words: Erythrina senegalensis, fabaceae, isoflavonoids, in vitro, antihepatotoxic, antioxidant