亚洲传统医药
亚洲传统医药

快速检索 年期检索

›› 2011, Vol. 6 ›› Issue (4): 168-174.

• Regular Articles • 上一篇    下一篇

Simultaneous determination of four effective compounds in Juejin Decoction by high performance liquid chromatography

  

  • 收稿日期:2010-05-02 修回日期:2010-07-29 出版日期:2011-08-20 发布日期:2011-08-20

Simultaneous determination of four effective compounds in Juejin Decoction by high performance liquid chromatography

Hongming Wang 1; Ning Liang 1 *; Xiaohong Hou 1; Guogang Zhang 2; Wei Chen 3
  

  1. 1. School of Pharmaceutical engineering, Shenyang Pharmaceutical University, Shenyang 110016, China; 2. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China; 3. School of Life Science And Biopharmceutics, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2010-05-02 Revised:2010-07-29 Online:2011-08-20 Published:2011-08-20
  • Contact: Ning Liang

Abstract: A simple method using high performance liquid chromatography coupled with ultraviolet (UV) detection was developed for the separation and determination of 5-Hydroxymethyl furfural, Ferulic acid, Cinnamaldehyde and Cinnamate acid in Juejin Decoction. The chromatography was performed on a C18 column at 30 °C, with a mobile phase composed of water containing 0.02% glacial acetic acid and methanol with gradient elution. The flow rate was 1.0 ml/min and UV detection wavelength was 282 nm. The linear ranges of the four compounds were 2.70-27.0 μg/ml (r=0.9996), 49.7-497 μg/ml (r=0.9995), 5.55-55.5 μg/ml (r=0.9996) and 0.359-3.59 μg/ml (r=0.9999), respectively. The recoveries of the four compounds were in the range of 98.2%-99.0%. The method is rapid, accurate and suitable for simultaneous determination of four effective compounds in Juejin Decoction.

Key words: Juejin Decoction, HPLC, 5-hydroxymethyl furfural, ferulic acid, cinnamaldehyde, cinnamate acid

版权所有 © 2019 《亚洲传统医药》

本系统由北京玛格泰克科技发展有限公司设计开发