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Indrani Bhattacharya, Kalpesh Ishnava*, Jenabhai Chauhan
Indrani Bhattacharya, Kalpesh Ishnava*, Jenabhai Chauhan
摘要： Oral diseases continue to be a major health problem worldwide. The increasing failure of chemotherapeutics and antibiotics resistance exhibited by pathogenic micro-organisms has led to the screening of several medicinal plants for their potential anticariogenic activity. In the present study, in vitro anticariogenic potential of the organic solvents of 20 plant leaves was evaluated by using five oral pathogen, Lactobacillus acidophilus, Lactobacillus casei, Staphylococcus aureus, Streptococcus mutans and Candida albicans. Agar well diffusion method and minimum inhibitory concentration were used for this purpose. Plant extracts exhibited varying degree of inhibited on growth of oral pathogen between hexane and ethyl acetate extracts is highly potential. Maximum activity show hexane (15mm) and ethyl acetate (16mm) extracts of Piper betle against S. mutans. The MIC of crude extract of ethyl acetate of Syzygium rubicundum against L. acidophilus was 0.12 mg/mL and ethyl acetate extract of Piper betle was 1 mg/mL and 0.25 mg/mL respectively for S. mutans and L. casei. The preliminary phytochemical analysis of crude extract of ethyl acetate of P. betle prsent the Phenolic compound and Alkaloid. Clear zone of inhibition against S. mutans located on TLC plate when bioautography performed using ethyl acetate extract of P. betle. The bioactive compound was separated from the crude ethyl acetate extract by using TLC technique. Further, spectroscopy analysis are requiring for determination of structure of bioactive compound. The finding may be useful in the development of drugs against oral pathogen.
