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›› 2014, Vol. 9 ›› Issue (1): 18-24.

• Regular Articles • 上一篇    

The effect of cuscuta chinensis seeds water extractionon promotion of proliferation, differentiation andmatrix mineralization of MC3T3-E1 cells

Xinyu Bai 1, Xiaofeng Yang 1, Ying Li 1, Jianmei Gao 2, Jianchun Li 1*   

  1. 1 Department of Pharmacology, Section of Shenyang Pharmaceutical University, 103 WenhuaRoad, Shenyang 110016, China;2 Department of Pharmacy, Zunyi Medical College, 201 Dalian Road, Zunyi 563000, China
  • 收稿日期:2013-06-21 修回日期:2013-07-20 出版日期:2014-02-20 发布日期:2014-02-20
  • 通讯作者: Jianchun Li

The effect of cuscuta chinensis seeds water extractionon promotion of proliferation, differentiation andmatrix mineralization of MC3T3-E1 cells

Xinyu Bai 1, Xiaofeng Yang 1, Ying Li 1, Jianmei Gao 2, Jianchun Li 1*   

  1. 1 Department of Pharmacology, Section of Shenyang Pharmaceutical University, 103 WenhuaRoad, Shenyang 110016, China;2 Department of Pharmacy, Zunyi Medical College, 201 Dalian Road, Zunyi 563000, China
  • Received:2013-06-21 Revised:2013-07-20 Online:2014-02-20 Published:2014-02-20
  • Contact: Jianchun Li

摘要： To study the influence of Cuscuta chinensis to the proliferation, differentiation and matrix mineralization of osteoblast
MC3T3-E1 cells, which is a valuable cell model in vitro to study drug effects on osteoblast, The proliferation promoting effect of
Cuscuta chinensis was determined by MTT assays. The differentiation of MC3T3-E1 cells was assisted by Cuscuta chinensis in
ALP active and collagen quantitative test Calcium deposition test implied the matrix mineralization of MC3T3-E1 cells. Western
Blot was used to investigate the expression of BMP-2 and Sirt3 protein. Different concentrations of Cuscuta chinensis (5-40 mg/L)
tested for 48 hours greatly promote the proliferation of MC3T3-E1 cells. Also, it increased the amount of collagen protein and
ALP activity. In addition, the matrix mineralization of MC3T3-E1 cells was increased, shown its in a time- and concentrationdependent
manner. In addition, the expression of BMP-2, Sirt3 and IDH2 proteins also enhanced significantly. From above
results, Cuscuta chinensis increased IDH2 protein by increasing the amount of BMP-2 protein and the expression of Sirt3 protein,
thereby to promote the proliferation, differentiation and matrix mineralization of MC3T3-E1 cells. The results demonstrated
that the future research of Cuscuta chinensis played a pivotal role to help reduce osteoporosis.

Abstract: To study the influence of Cuscuta chinensis to the proliferation, differentiation and matrix mineralization of osteoblast
MC3T3-E1 cells, which is a valuable cell model in vitro to study drug effects on osteoblast, The proliferation promoting effect of
Cuscuta chinensis was determined by MTT assays. The differentiation of MC3T3-E1 cells was assisted by Cuscuta chinensis in
ALP active and collagen quantitative test Calcium deposition test implied the matrix mineralization of MC3T3-E1 cells. Western
Blot was used to investigate the expression of BMP-2 and Sirt3 protein. Different concentrations of Cuscuta chinensis (5-40 mg/L)
tested for 48 hours greatly promote the proliferation of MC3T3-E1 cells. Also, it increased the amount of collagen protein and
ALP activity. In addition, the matrix mineralization of MC3T3-E1 cells was increased, shown its in a time- and concentrationdependent
manner. In addition, the expression of BMP-2, Sirt3 and IDH2 proteins also enhanced significantly. From above
results, Cuscuta chinensis increased IDH2 protein by increasing the amount of BMP-2 protein and the expression of Sirt3 protein,
thereby to promote the proliferation, differentiation and matrix mineralization of MC3T3-E1 cells. The results demonstrated
that the future research of Cuscuta chinensis played a pivotal role to help reduce osteoporosis.

Key words: Cuscuta chinensis, osteoblast, proliferation, differentiation, matrix mineralization

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