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The effect of cuscuta chinensis seeds water extractionon promotion of proliferation, differentiation andmatrix mineralization of MC3T3-E1 cells
Xinyu Bai;Xiaofeng Yang;Ying Li;Jianmei Gao;Jianchun Li *
2014 (1):
18-24.
摘要
(
350 )
To study the influence of Cuscuta chinensis to the proliferation, differentiation and matrix mineralization of osteoblast MC3T3-E1 cells, which is a valuable cell model in vitro to study drug effects on osteoblast, The proliferation promoting effect of Cuscuta chinensis was determined by MTT assays. The differentiation of MC3T3-E1 cells was assisted by Cuscuta chinensis in ALP active and collagen quantitative test Calcium deposition test implied the matrix mineralization of MC3T3-E1 cells. Western Blot was used to investigate the expression of BMP-2 and Sirt3 protein. Different concentrations of Cuscuta chinensis (5-40 mg/L) tested for 48 hours greatly promote the proliferation of MC3T3-E1 cells. Also, it increased the amount of collagen protein and ALP activity. In addition, the matrix mineralization of MC3T3-E1 cells was increased, shown its in a time- and concentrationdependent manner. In addition, the expression of BMP-2, Sirt3 and IDH2 proteins also enhanced significantly. From above results, Cuscuta chinensis increased IDH2 protein by increasing the amount of BMP-2 protein and the expression of Sirt3 protein, thereby to promote the proliferation, differentiation and matrix mineralization of MC3T3-E1 cells. The results demonstrated that the future research of Cuscuta chinensis played a pivotal role to help reduce osteoporosis.
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