亚洲传统医药
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›› 2014, Vol. 9 ›› Issue (2): 34-44.

• Regular Articles • 上一篇    下一篇

Development and validation of HPTLC methodfor estimation of imperatorin in anti-obesity polyherbalformulation, Safoof-e-Muhazzil and some of its ingredients

Kamran Javed Naquvi 1*, S. H. Ansari 1, Mohd. Ali 1, A. K. Najmi 2, Javed Ahamad 1   

  1. 1 Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard,New Delhi 110062, India;2 Department of Pharmacology, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India
  • 收稿日期:2013-12-08 修回日期:2014-04-14 出版日期:2014-04-20 发布日期:2014-04-20
  • 通讯作者: Kamran Javed Naquvi

Development and validation of HPTLC methodfor estimation of imperatorin in anti-obesity polyherbalformulation, Safoof-e-Muhazzil and some of its ingredients

Kamran Javed Naquvi 1*, S. H. Ansari 1, Mohd. Ali 1, A. K. Najmi 2, Javed Ahamad 1   

  1. 1 Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard,New Delhi 110062, India;2 Department of Pharmacology, Faculty of Pharmacy, Jamia Hamdard, New Delhi 110062, India
  • Received:2013-12-08 Revised:2014-04-14 Online:2014-04-20 Published:2014-04-20
  • Contact: Kamran Javed Naquvi

摘要： Furanocoumarins are the coumarin derivatives distributed abundantly in higher plants. They can be grouped into the linear type
and angular type. Imperatorin belongs to linear furanocoumarin class of compounds. It is reported to play a vital role in many
biological activities. Therefore, a high-performance thin layer chromatography (HPTLC) densitometric method was developed
and validated for the estimation of imperatorin. Imperatorin was separated on aluminium-backed silica gel 60 F254 plates using
toluene:ethyl acetate (9:1, % v/v) as the mobile phase. This system was found to give a compact band of imperatorin at Rf
value 0.49 ± 0.02. The limit of detection and limit of quantification were found to be 35.14 and 105.57 ng/spot, respectively. The
proposed method was employed with a high degree of precision and accuracy for the estimation of imperatorin in Nardostachys
jatamansi DC, Rosa damascena Mill., Apium graveolens Linn., Trachyspermum ammi Linn. and in-house Unani formulation
Safoof-e-Muhazzil, containing these plants as ingredients.

Abstract: Furanocoumarins are the coumarin derivatives distributed abundantly in higher plants. They can be grouped into the linear type
and angular type. Imperatorin belongs to linear furanocoumarin class of compounds. It is reported to play a vital role in many
biological activities. Therefore, a high-performance thin layer chromatography (HPTLC) densitometric method was developed
and validated for the estimation of imperatorin. Imperatorin was separated on aluminium-backed silica gel 60 F254 plates using
toluene:ethyl acetate (9:1, % v/v) as the mobile phase. This system was found to give a compact band of imperatorin at Rf
value 0.49 ± 0.02. The limit of detection and limit of quantification were found to be 35.14 and 105.57 ng/spot, respectively. The
proposed method was employed with a high degree of precision and accuracy for the estimation of imperatorin in Nardostachys
jatamansi DC, Rosa damascena Mill., Apium graveolens Linn., Trachyspermum ammi Linn. and in-house Unani formulation
Safoof-e-Muhazzil, containing these plants as ingredients.

Key words: Furanocoumarins, imperatorin, marmelosin, Safoof-e-Muhazzil, ICH guidelines

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