Simultaneous quantification of three major sanshools
in analgesic bacteriostatic gel by HPLC

亚洲传统医药

• Regular Article • 上一篇

Simultaneous quantification of three major sanshools in analgesic bacteriostatic gel by HPLC

Rong Rong, Meiyan Zhang, Xianying Zhou, Qili Zhang, Zhiguo Yu, Yunli Zhao*

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China

收稿日期:2015-03-23 修回日期:2016-04-08 出版日期:2016-04-20 发布日期:2016-04-08

Simultaneous quantification of three major sanshools in analgesic bacteriostatic gel by HPLC

Rong Rong, Meiyan Zhang, Xianying Zhou, Qili Zhang, Zhiguo Yu, Yunli Zhao*

School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China

Received:2015-03-23 Revised:2016-04-08 Online:2016-04-20 Published:2016-04-08
Contact: Yunli Zhao*, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, Liaoning 110016, China; Tel: +86-24-23986295; E-mail: yunli76@163.com

摘要/Abstract

摘要： A rapid and sensitive high-performance liquid chromatographic (HPLC) method had been developed and validated for the analysis of three main ingredients in analgesic bacteriostatic gel. Hydroxy-α-sanshool (HAS), hydroxyl-β-sanshool (HBS) and hydroxy-γ-sanshool (HRS) were separated on a Thermo Hypersil GOLD C18 column (150 mm × 4.6 mm, 5 μm) with gradient elution system. The mobile phase consisted of acetonitrile-water (v/v) with a flow rate of 1.0 mL/min and UV detection was performed at 270 nm. The method was validated according to the Chinese Pharmacopeia (2015). Linearity (r > 0.9990) was observed over the concentration ranges of 3.24–58.32, 0.656–11.81 and 0.304–5.48 mg/L for HAS, HBS and HRS. The average recovery for HAS, HBS and HRS was 96.9%, 97.2% and 97.9%. The method was found simple and rapid, which can be suitable for the determination of the three main ingredients in analgesic bacteriostatic gel.

关键词: analgesic bacteriostatic gel, sanshools, Method validation, HPLC

Abstract: A rapid and sensitive high-performance liquid chromatographic (HPLC) method had been developed and validated for the analysis of three main ingredients in analgesic bacteriostatic gel. Hydroxy-α-sanshool (HAS), hydroxyl-β-sanshool (HBS) and hydroxy-γ-sanshool (HRS) were separated on a Thermo Hypersil GOLD C18 column (150 mm × 4.6 mm, 5 μm) with gradient elution system. The mobile phase consisted of acetonitrile-water (v/v) with a flow rate of 1.0 mL/min and UV detection was performed at 270 nm. The method was validated according to the Chinese Pharmacopeia (2015). Linearity (r > 0.9990) was observed over the concentration ranges of 3.24–58.32, 0.656–11.81 and 0.304–5.48 mg/L for HAS, HBS and HRS. The average recovery for HAS, HBS and HRS was 96.9%, 97.2% and 97.9%. The method was found simple and rapid, which can be suitable for the determination of the three main ingredients in analgesic bacteriostatic gel.

Key words: analgesic bacteriostatic gel, sanshools, Method validation, HPLC

. Simultaneous quantification of three major sanshools in analgesic bacteriostatic gel by HPLC[J]. 亚洲传统医药.

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Simultaneous quantification of three major sanshools in analgesic bacteriostatic gel by HPLC

Simultaneous quantification of three major sanshools in analgesic bacteriostatic gel by HPLC

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