Isolation and identification of chemical constituents from the vinegar-prepared Corydalis yanhusuo

亚洲传统医药 ›› 2021, Vol. 16 ›› Issue (4): 225-233.

Isolation and identification of chemical constituents from the vinegar-prepared Corydalis yanhusuo

出版日期:2021-08-25 发布日期:2021-09-02

Isolation and identification of chemical constituents from the vinegar-prepared Corydalis yanhusuo

Online:2021-08-25 Published:2021-09-02

摘要/Abstract

摘要： Phytochemical investigation of the vinegar-prepared Corydalis yanhusuo led to the isolation of one aristolactam derivative, 1,2,8,9-tetramethoxy-5-methyldibenzo[cd,f]indol-4-(5H)-one (1), and seven aporphine alkaloids, including 2,9,10-trimethoxydibenz[de,g]quinolin-7-one (2), 1-hydroxy-2,9,10-trimethoxy-7H-dibenzo(de,g)quinoline-7-one (3), oxoglaucine (4), N-methyloxoglaucine trifluoroacetate trifluoroacetate (5), corunine acetate (6), pontevedrine (7), and oxoglaucidaline trifluoroacetate (8). The structures of the isolated compounds were elucidated by extensive spectroscopic data analysis and comparison with the previous reports. Among them, compounds 1 and 2 were obtained as natural products for the first time, and their NMR data were unambiguously assigned. In addition, compound 1 exhibited moderate cytotoxic activity against HepG2 cells with an IC50 value of 16.0 ± 6.4 μM.

关键词: Corydalis yanhusuo, isolation and purification, alkaloids, cytotoxic activity

Abstract: Phytochemical investigation of the vinegar-prepared Corydalis yanhusuo led to the isolation of one aristolactam derivative, 1,2,8,9-tetramethoxy-5-methyldibenzo[cd,f]indol-4-(5H)-one (1), and seven aporphine alkaloids, including 2,9,10-trimethoxydibenz[de,g]quinolin-7-one (2), 1-hydroxy-2,9,10-trimethoxy-7H-dibenzo(de,g)quinoline-7-one (3), oxoglaucine (4), N-methyloxoglaucine trifluoroacetate trifluoroacetate (5), corunine acetate (6), pontevedrine (7), and oxoglaucidaline trifluoroacetate (8). The structures of the isolated compounds were elucidated by extensive spectroscopic data analysis and comparison with the previous reports. Among them, compounds 1 and 2 were obtained as natural products for the first time, and their NMR data were unambiguously assigned. In addition, compound 1 exhibited moderate cytotoxic activity against HepG2 cells with an IC50 value of 16.0 ± 6.4 μM.

Key words: Corydalis yanhusuo, isolation and purification, alkaloids, cytotoxic activity

Guiyang Xia, Dongjie Fang, Yuzhuo Wu, Huan Xia, Xinlan Yu, Sheng Lin, Hongcai Shang. Isolation and identification of chemical constituents from the vinegar-prepared Corydalis yanhusuo[J]. 亚洲传统医药, 2021, 16(4): 225-233.

[1]	Sihui Mi, Rui Guo, Chuang Xie, Xiaoxiao Huang. Compounds from the flowers and fruits of Abelmoschus esculentus (L.) Moench[J]. 亚洲传统医药, 2021, 16(5): 269-275.
[2]	Zhiyong Xu, Yufei Xi, Xiaoxiao Huang, Shaojiang Song. Research progress on the main chemical constituents and pharmacological effects of Piper betle L.[J]. 亚洲传统医药, 2021, 16(5): 294-308.
[3]	Ming Qu, Ying Wang, Shijie Cao, Yalu Liu, Da Liu, Feng Qiu, Ning Kang. Main alkaloids of Rhizoma Coptidis improved palmitic acidinduced insulin resistance in HepG2 cells via AMPK and MAPK signaling pathway[J]. 亚洲传统医药, 2020, 15(5): 239-254.
[4]	Weiyu Zhou, Ming Bai, Xiaoxiao Huang, Shaojiang Song. Network pharmacology-based virtual screening of the active ingredients and potential targets from amide alkaloids of Piper genus against Alzheimer’s disease[J]. 亚洲传统医药, 2018, 13(5): 184-191.
[5]	Weiyu Zhou, Ming Bai, Xiaoxiao Huang, Shaojiang Song. Amide Alkaloids from the genus Piper: A Review of its Phytochemistry[J]. 亚洲传统医药, 2018, 13(3): 110-126.
[6]	. Antibacterial Activity Test of Coptis chinensis Alkaloids Combined with Moxifloxacin[J]. 亚洲传统医药, 2016, 11(4): 99-104.
[7]	Sunil Kumar Mishra *;Paras Nath Singh;Satyadev Dubey . Analgesic, anti-inflammatory and antipyretic activity of Capparis zeylanica Linn. root extract[J]. , 2013, 8(2): 30-39.
[8]	Varadharajan Madhavan;Sunkam Yoganarasimhan ;Poonam Shah;Magadi Gurudeva;Rajamanickam Deveswaran . Pharmacognostical studies on the root of Toddalia asiatica* (L.) Lam. (Rutaceae)[J]. , 2012, 7(5): 195-203.

亚洲传统医药

Isolation and identification of chemical constituents from the vinegar-prepared Corydalis yanhusuo

Isolation and identification of chemical constituents from the vinegar-prepared Corydalis yanhusuo

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 8

编辑推荐

Metrics

本文评价