Asian Journal
Of Traditional Medicines

Abstract:

In present work, simultaneous quantitative estimation of two biologically active flavonoidal compounds, quercetin and rutin, in Tephrosia purpurea (TP) leaves was performed using high-performance thin-layer chromatography (HPTLC). TLC aluminum plates precoated with silica-gel RP-18 F 254 S were used with a mobile phase of methanol-water-formic acid (40:57:3, v/v/v) (reported earlier for another herbal drug) and densitometric determination of these compounds was carried out at 254 nm in reflectance/absorbance mode. The linear regression data for the calibration plots showed a good linear relationship with r=0.9942 and 0.9996 for quercetin and rutin, respectively. A recovery study was carried out to check the accuracy of the method. At two different levels the average recovery of quercetin and rutin was 100.16 % and 99.94 %, respectively. The present method has been repeated with some modification in an earlier reported method and the present method can be used routinely for the quantitative estimation of these two compounds.

Key words: HPTLC, quantitative estimation, quercetin, rutin, Tephrosia purpurea