Asian Journal
Of Traditional Medicines

Abstract: High performance liquid chromatography coupled with diode array detection (HPLC-DAD) was developed to evaluate the quality of Radix Glehniae. Chromatographic fingerprints and the amounts of coumarins and polyacetylenes were determined in Radix Glehniae collected from different regions of China. The analysis was carried out on a Prevail C₁₈ analytical column (250 mm × 4.6 mm id. 5 µm) using linear gradient elution of acetonitrile-water. The correlation coefficients of similarity were determined and twelve common peaks were defined from the HPLC fingerprints: five of the peaks were identified as psoralen, imperatorin, isoimperatorin, falcarindiol and falcarinol. The contents of these five identified compounds were subsequently determined by the validated HPLC-DAD method with high sensitivity, precision, repeatability and accuracy. This newly developed method involving a combination of chromatographic fingerprinting and quantitative analysis is suitable for the quality of Radix Glehniae.

Key words: HPLC-DAD, quality evaluation, coumarin, polyacetylene, Radix Glehniae