›› 2010, Vol. 5 ›› Issue (2): 70-74.
• Regular Articles • Previous Articles Next Articles
Tianhong Zhang; Dan Yang; Yuanguo Wang; Xiaohun Chen; Kaishun Bi *
Received:
Revised:
Online:
Published:
Contact:
Abstract: A high performance liquid chromatographic method was developed for the simultaneous quantification of four major ingredients in the Sumbucus chinensis Lindl., namely chlorogenic acid, caffeic acid, p-coumaric acid and ferulaic acid. The chromatographic separation was performed on a phenomenex C18 column (250×4.6mm, 5 µm) with gradient elution using methanol-0.05 % formic acid in water, and a detector wavelength of 326 nm. The linear ranges of the four compounds were 1.552-15.52 μg.ml-1 (r=0.9998), 0.0691-0.691 μg.ml-1 (r=0.9996), 0.148-1.48 μg.ml-1 (r=0.9997) and 0.174-1.74 μg.ml-1 (r= 0.9997), respectively. The recoveries of the four compounds were in the range 98.2 %-100. 7 %. The results indicated that this developed assay method was rapid, accurate, and reliable and could be readily used as a quality control method for the Sumbucus chinensis Lindl.
Key words: Sumbucus chinensis Lindl., HPLC, quality control
Tianhong Zhang;Dan Yang;Yuanguo Wang;Xiaohun Chen;Kaishun Bi *. Simultaneous determination of four components in the herbs of Sumbucus chinensis Lindl. by high performance liquid chromatography[J]. , 2010, 5(2): 70-74.
0 / / Recommend
Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks
URL: http://asianjtm.syphu.edu.cn/EN/
http://asianjtm.syphu.edu.cn/EN/Y2010/V5/I2/70