Asian Journal
Of Traditional Medicines

Abstract: A simple and reliable high-performance liquid chromatographic method was established for the simultaneous determination of
myricetin, quercetin, and kaempferol in Juglandis Mandshuricae Cortex. The chromatographic separation was performed on a
Kromasil C18 column (250 mm×4.6 mm, 5 μm) with a gradient elution of methanol and 0.2% aqueous formic acid at a flow rate
of 1.0 ml/min. The detector wavelength was 370 nm and the column temperature was 30ºC. The linear ranges were from 3.93 to
62.88 μg/ml (r=0.9998), 3.80 to 60.80 μg/ml (r=0.9996), and 0.41 to 6.62 μg/ml (r=0.9997) for myricetin, quercetin, and kaempferol,
respectively. The precision for myricetin, quercetin, and kaempferol was 1.8%, 0.4%, and 1.9%. The method recoveries were
101.0% for myricetin, 96.5% for quercetin, and 97.0% for kaempferol. The contents of the target analytes were (98.90±0.45) μg/g
for myricetin, (54.60±0.30) μg/g for quercetin, and (7.20±0.07) μg/g for kaempferol. The results demonstrated that the developed
HPLC method would be employed to control the quality of Juglandis Mandshuricae Cortex.

Key words: Juglandis Mandshuricae Cortex, myricetin, quercetin, kaempferol, HPLC