Asian Journal
Of Traditional Medicines

Abstract: This paper is to investigate the purification of an antibacterial effective fraction from Syringae Folium using macroporous
resins. Static adsorption and desorption tests were carried out for the screening of macroporous resins. A desorption experiment
was performed on selected D101 resin to optimize the separation process. The effects of several parameters, including the elution
flow rate, and ratio of the diameter to the height of the bed, were examined in an orthogonal experiment. The results obtained
demonstrated that the optimized conditions, i.e. an elution solution of 55% ethanol, an absorption flow rate of 1 bed volume (BV) /h,
an elution flow rate of 5 BV/h and etc. The content of the antibacterial effective component was over 65% after purification using
D101 resin, indicating that the present method is suitable for the large-scale preparation of an antibacterial effective fraction
from Syringae Folium.

Key words: syringae folium, purification, effective fraction, macroporous resins