27 Fingerprint analysis of Clerodendranthus Spicatus
from different regions by high-performance liquid
chromatography

27 Fingerprint analysis of Clerodendranthus Spicatus from different regions by high-performance liquid chromatography

Song Xue 1, Ran Zhang 1, Jing Su 1, Xuelan Li 2, Mingfeng Qiu 1*, Yingzi Wang 3*

1 School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China; 2 Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Jinghong 666100, China; 3 School of Chinese Material, Beijing University of Traditional Chinese Medicine, Beijing 100102, China

Received:2015-05-06 Revised:2015-10-20 Online:2016-02-20 Published:2015-10-20
Contact: Mingfeng Qiu, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China; Tel.: 13917181875; E-mail: mfqiu@ sjtu.edu.cn; Yingzi Wang, School of Chinese Medicine, Beijing University of Traditional Chinese Medicine, Beijing, 100102, China; Tel.: 13717529199; E-mail:wangyzi@sina.com

Abstract

Abstract: AIM: To develope a novel, simple and accurate fingerprint method for the quality control of Clerodendranthus Spicatus from different regions by high-performance liquid chromatography, which is widely used for urolithiasis. METHODS: Chromatographic separation was performed on Agilent SB-C18 (250 mm×4.6 mm, 5 μm) by the gradient elution of 0.1% H3PO4 in water and acetonitrile at 0.8 mL·min-1 at 210 nm by DAD detector. The column temperature was set at 30 °C. RESULTS: The method has good stability and reproducibility with RSD less than 2%. 10 peaks were selected as the common peaks. Rosmarinic acid was taken as a reference. The similarity of 10 batches was above 0.90. CONCLUSION: This method is simple and specific, which provided an effective way to assess the quality of Clerodendranthus Spicatus.

Key words: Clerodendranthus Spicatus, fingerprint, quality control

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