Molecular compliance of the responses of different Ginseng to oxidative stress

Molecular compliance of the responses of different Ginseng to oxidative stress

Sainan Meng 1?, Xia Chen 1?, Bo Yuan 2, Jincai Lu 3, Haiyan Xu 2, Xiang Zhao 4, Feng Xu 1*

1 Department of Physiology, Shenyang Pharmaceutical University, Shenyang, 110016, China; 2 Department ofPharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China; 3 Department of Phytochemistry, Shenyang Pharmaceutical University, Shenyang, 110016, China; 4 Department of Medicinal Chemistry, Shenyang Pharmaceutical University, Shenyang, 110016, China

Received:2016-05-01 Revised:2017-03-21 Online:2017-06-20 Published:2017-03-21
Contact: Department of Physiology, School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang,110016, People’s Republic of China (Feng Xu); Tel.: +86-24-23986265; Fax: +86-24-23986265; E-mail: xufengyaoli@ sina.com (Feng Xu)

Abstract

Abstract: The content of ginsenosides and antioxidative activity of different ginseng extracts were investigated to explore the molecular compliance of antioxidative activity with ginsenosides in wild ginseng, transplant wild ginseng and cultivated ginseng in this research. The results of TLC and HPLC-MS showed that three different ginseng extracts contain the same kinds of ginsenosides, however, the content of ginsenoside Re is significantly different. All three kinds of ginseng extracts protected PC12 cells from H2O2-induced oxidative injury. Three kinds of ginseng extracts increased the levels of intracellular glutathione peroxidase (GSH-PX) and total-superoxide dismutase (T-SOD), decreased intracellular reactive oxygen species (ROS) generation and Malondialdehyde (MDA) production. The extract of wild ginseng exhibited strongest modulation on cell viability and GSH-PX activity, which is consistent with the difference of Re content in three ginseng extracts. The difference in antioxidant activity may be closely related to the content of ginsenoside Re in three ginseng extracts.

Key words: ginseng, ginsenosides, oxidative stress, antioxidants

Sainan Meng, Xia Chen, Bo Yuan, Jincai Lu, Haiyan Xu, Xiang Zhao, Feng Xu. Molecular compliance of the responses of different Ginseng to oxidative stress[J]. Asina Journal Of Traditional Medicines.

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