Asian Journal
Of Traditional Medicines

Abstract: A low cost, rapid and sensitive preparation method of silica gel supported ionic liquid (SGSIL) combined with matrix solid phase
dispersion (MSPD) followed by high performance liquid chromatography (HPLC) with ultraviolet detection (UV) is proposed,
and it was applied to determine the seven active compounds in Salvia Miltiorrhiza herb. SGSIL and ionic liquid [BMIM]BF4 were used as the adsorbent and the green elution reagent in the MSPD procedure. Several extraction conditions including type of filler and elution solvent, the volume of elution solvent, material liquid ratio were optimized. Under the optimum conditions, the
SGSIL-MSPD-HPLC method showed a low limit of detection (LOD, S/N = 3) of 0.0122-0.8788 μg/mL for standard solution, limit
of quantification (LOQ, S/N = 10) of 0.0406-2.9292 μg/mL for standard solution, wide linear range from 1.56 to 2000 μg/mL for all compounds for standard solution, correlation coefficients (r) of more than 0.9990, acceptable reproducibility (relative standard deviations, RSDs < 3.54%), and precision of RSDs < 3.36% for intra-day, RSDs < 3.50% for inter-day. The satisfactory recoveries ranged from 96.4 to 102.5, with RSDs less than 3.45%. The developed SGSIL-MSPD method is easier and more suitable for the determination of the seven active compounds in Salvia Miltiorrhiza herb than the traditional ultrasonic extraction. It was an effective and efficient method for the extraction and quantification of the seven active compounds in traditional Chinese herbal samples.

Key words: high performance liquid chromatography (HPLC), silica gel supported ionic liquid (SGSIL), matrix solid phase dispersion
(MSPD), Salvia miltiorrhiza (SM), determination