High performance thin layer chromatography as an effective tool for rapid screening of 5α-reductase inhibitors

Abstract

Abstract: Benign prostatic hyperplasia (BPH) is a common disease in men, and is known to be related to 5α-reductase, which can affect steroid metabolism. Under the promotion of 5α-reductase, testosterone can be converted into dihydrotestosterone (DHT), and excessive DHT will cause related diseases. Since BPH seriously affects the quality of life of patients, it is essential to discover effective 5α-reductase inhibitors. In this study, the simple analytical method of high performance thin layer chromatography (HPTLC) was used to screen active compounds, and seven compounds with strong activity were screened out. This research will provide a reference for studying the material basis of drugs for the treatment of BPH.

Key words: 5α-reductase inhibitor, benign prostatic hyperplasia, HPTLC, steroid hormones, dihydrotestosterone

Ruirong Zheng†, Mooseob Kim†, Zhengtao Wang, Li Yang. High performance thin layer chromatography as an effective tool for rapid screening of 5α-reductase inhibitors[J]. Asian Journal of Traditional Medicines, 2021, 16(4): 216-224.

Trendmd

[1]	Ajay Kumar Meena, R. Ilavarasan, Arjun Singh, Lavkush Sharma, Amit Dixit, N. Srikanth. Identification and quantitative estimation of Emodin in Rhei Emodi Wall. Rhizome and the relevant polyherbal ayurvedic formulation [J]. Asian Journal of Traditional Medicines, 2021, 16(6): 351-366.
[2]	Lavanya Alagusolaiyan;Shakila Ramachandran *;Sasikala Ethirajulu;Ayyasamy Sengan . Pharmacognostical studies on the leaves ofCanavalia virosa (Roxb.) Wight & Arn. [J]. , 2014, 9(1): 8-17.
[3]	Ilanchezhian Rangasamy *;Rabinarayan Acharya;Roshy J Chowallur;Vinay J Shukla . Shodhana (purificatory procedures) of Bhallataka (Semecarpusanacardium Linn.) fruit by traditional frying method [J]. , 2014, 9(1): 1-7.
[4]	Varadharajan Madhavan;Sunkam Yoganarasimhan *;Rajendra Prasad;Magadi Gurudeva;Rajamanickam Deveswaran . Pharmacognostical studies on the leaves ofBasilicum polystachyon Moench [J]. , 2013, 8(1): 11-19.
[5]	Varadharajan Madhavan ;Sunkam Yoganarasimhan ;Magadi Gurudeva;Ajay Shankar Pandey;Deveswaran Rajamanickam . Pharmacognostical studies of the root of Capparis sepiaria* L. (Himsra) - an ayurvedic drug [J]. , 2012, 7(5): 226-235.
[6]	Varadharajan Madhavan;Sunkam Yoganarasimhan ;Poonam Shah; Magadi Gurudeva;Rajamanickam Deveswaran . Pharmacognostical studies on the root of Toddalia asiatica* (L.) Lam. (Rutaceae) [J]. , 2012, 7(5): 195-203.
[7]	Varadharajan Madhavan;Azharali Mulla Khan ;Sunkam Yoganarasimhan ;Magadi Gurudeva ;Deveswaran Rajamanickam . Pharmacognostical studies on the root and rhizome of Cryptocoryne spiralis* (Retz.) Fisch. ex Wydl. (Araceae) - Alternate source for Tagara [J]. , 2012, 7(3): 118-128.
[8]	Varadharajan Madhavan;Anita Murali ;Sunkam Yoganarsimhan;Ajay Shankar Pandey . Protective effect of the roots of Capparis sepiaria* Linn. (Himsra) on carbon tetrachloride-induced hepatotoxicity [J]. , 2012, 7(1): 18-28.
[9]	Varadharajan Madhavan;Usha Munisamy;Sunkam Yoganarasimhan ;Magadi Gurudeva;Deveswaran Rajamanickam;Sundara Saravanan;Rajendran Mythreyi . Pharmacognostical studies on the roots of Maerua oblongifolia* (Forsk.) A. Rich. (Capparaceae) [J]. , 2012, 7(1): 29-38.
[10]	Varadharajan Madhavan;Dinesh Kumar Yadav;Magadi Gurudeva;Sunkam Yoganarasimhan *. Pharmacognostical studies on the leaves of Colebrookea oppositifolia Smith [J]. , 2011, 6(4): 134-144.
[11]	Akkula Viswanath;Karthik Mahalingam;Satish Kumar Uppala;Ravichandiran Velayudam;K. F. H. Nazeer Ahamed *. Comparative evaluation of antioxidant activity of some indian traditional medicinal plants [J]. , 2011, 6(4): 154-162.
[12]	Patel Sonal;Zaveri Maitreyi . Pharmacognostic study of the root of Justicia gendarussa Burm [J]. , 2011, 6(2): 61-72.
[13]	V Madhavan;Shilpi Arora;S N Yoganarasimhan ;M R Gurudeva. Pharmacognostical studies on the rhizome and roots of Nymphoides indica* (L.) Ktze. – alternate source for Tagara [J]. , 2011, 6(1): 14-25.
[14]	Avijeet Jain ;Santram Lodhi;A K Singhai. Simultaneous estimation of quercetin and rutin in Tephrosia purpurea* Pers by high performance thin-layer chromatography [J]. , 2009, 4(3): 104-109.
[15]	V. Madhavan;Jagadish Chandra Nagar;Mythreyi R.;Gurudeva M. R.; S. N. Yoganarasimhan * . Pharmacognostical studies on the adventitious roots of Pandanus fascicularis Lam. (Pandanaceae) [J]. , 2009, 4(2): 58-65.

Asian Journal
Of Traditional Medicines