Isolation and identification of chemical constituents from the vinegar-prepared Corydalis yanhusuo

Abstract

Abstract: Phytochemical investigation of the vinegar-prepared Corydalis yanhusuo led to the isolation of one aristolactam derivative, 1,2,8,9-tetramethoxy-5-methyldibenzo[cd,f]indol-4-(5H)-one (1), and seven aporphine alkaloids, including 2,9,10-trimethoxydibenz[de,g]quinolin-7-one (2), 1-hydroxy-2,9,10-trimethoxy-7H-dibenzo(de,g)quinoline-7-one (3), oxoglaucine (4), N-methyloxoglaucine trifluoroacetate trifluoroacetate (5), corunine acetate (6), pontevedrine (7), and oxoglaucidaline trifluoroacetate (8). The structures of the isolated compounds were elucidated by extensive spectroscopic data analysis and comparison with the previous reports. Among them, compounds 1 and 2 were obtained as natural products for the first time, and their NMR data were unambiguously assigned. In addition, compound 1 exhibited moderate cytotoxic activity against HepG2 cells with an IC50 value of 16.0 ± 6.4 μM.

Key words: Corydalis yanhusuo, isolation and purification, alkaloids, cytotoxic activity

Guiyang Xia, Dongjie Fang, Yuzhuo Wu, Huan Xia, Xinlan Yu, Sheng Lin, Hongcai Shang. Isolation and identification of chemical constituents from the vinegar-prepared Corydalis yanhusuo[J]. Asian Journal of Traditional Medicines, 2021, 16(4): 225-233.

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