Asian Journal of Traditional Medicines
Asian Journal
Of Traditional Medicines

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›› 2006, Vol. 1 ›› Issue (3-4): 159-165.

• 论文 • Previous Articles     Next Articles

Capsaicin Induces Apoptosis in HeLa Cells via Bax/Bcl-2 and Caspase-3 Pathways

Wei Yang1,2; Xianfeng Gong2,3; Xueling Zhao3; Weiwei An2; Xisha Wang2,4; Minwei Wang2*   

  1. 1 Pharmacological Lab. of Pharmaceutical Industrial Research Institute in Guangzhou, Guangzhou 510240, China; 2 Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang 110016, China; 3Department of Pharmaceutical Engineering, Heilongjiang University, Harbin 150080, China; 4 Department of Pharmacology, Guangdong Pharmaceutical University, Guangzhou 510006, China
  • Received:2006-01-01 Revised:2006-01-01 Online:2006-10-15 Published:2006-10-15
  • Contact: Minwei Wang

Abstract: The mechanisms of capsaicin-induced apoptosis on HeLa cells wree studied. MTT cell growth assay, fluorescence microscopical observation, DNA agarose gel electrophoresis, flow cytometry and western blot analysis were carried out. Capsaicin induced HeLa cell death in a time- and dose-dependent manner. Capsaicin-treated HeLa cells showed typical characteristics of apoptosis including the morphologic changes, DNA fragmentation and sub-diploid peak. Caspase-3 inhibitor, z-DEVD-fmk, partially prevented capsaicin-induced apoptosis. Protein expression
of procaspase-3 and one of its substrates, inhibitor of caspase activated DNase ( ICAD ) was decreased in the capsaicin-treated cells. Bcl-2
expression was down-regulated, whereas Bax expression was increased. P53 protein expression was also increased. Capsaicin induced HeLa cell apoptosis through activation of caspase cascade, up-regulation of Bax/Bcl-2 ratio and p53 pathways.

Key words: Capsaicin, Apoptosis, HeLa

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