亚洲传统医药
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›› 2015, Vol. 10 ›› Issue (4): 79-84.

• 论文 •    下一篇

Determination of (-)-secoisolariciresinol 4-O-β-Dglucopyranoside in Glehnia littoralis by SPE-HPLC

Na Liu, Hongyu Ma, Fang Gao, Zhong Yuan*   

  1. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People’s Republic of China
  • 收稿日期:2015-01-15 修回日期:2015-07-12 出版日期:2015-08-20 发布日期:2015-08-20

Determination of (-)-secoisolariciresinol 4-O-β-Dglucopyranoside in Glehnia littoralis by SPE-HPLC

Na Liu, Hongyu Ma, Fang Gao, Zhong Yuan*   

  1. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, People’s Republic of China
  • Received:2015-01-15 Revised:2015-07-12 Online:2015-08-20 Published:2015-08-20
  • Contact: Zhong Yuan

摘要： A new simple and reliable HPLC method was established for determining the contents of (-)-secoisolariciresinol 4-O-β-Dglueopyranoside in Glehnia littoralis. HPLC analysis was performed on Classic C18-SPE column (500 mg, 6 ml) and C18 column (200 mm×4.6 mm, 5 μm) using a gradient elution of water (A) and methanol-acetonitrile (B, 10:90, v/v), the column temperature was set at 30°C, the flow rate was 1.0 ml·min-1 and the detection wavelength was at 230 nm. The linear range was 10.7- 214.0 μg·ml-1, the average recovery was 96.1%, and RSD was 1.2%. The method was reliable, accurate, and was successfully applied to quantify the (-)-secoisolariciresinol 4-O-β-D-glucopyranoside content in Glehnia littoralis collected from different areas in China.

Abstract: A new simple and reliable HPLC method was established for determining the contents of (-)-secoisolariciresinol 4-O-β-Dglueopyranoside in Glehnia littoralis. HPLC analysis was performed on Classic C18-SPE column (500 mg, 6 ml) and C18 column (200 mm×4.6 mm, 5 μm) using a gradient elution of water (A) and methanol-acetonitrile (B, 10:90, v/v), the column temperature was set at 30°C, the flow rate was 1.0 ml·min-1 and the detection wavelength was at 230 nm. The linear range was 10.7- 214.0 μg·ml-1, the average recovery was 96.1%, and RSD was 1.2%. The method was reliable, accurate, and was successfully applied to quantify the (-)-secoisolariciresinol 4-O-β-D-glucopyranoside content in Glehnia littoralis collected from different areas in China.

Key words: determination, Glehnia littoralis, SPE-HPLC, (-)-secoisolariciresinol 4-O-β-D-glucopyranoside

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