亚洲传统医药
亚洲传统医药

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›› 2015, Vol. 10 ›› Issue (4): 91-96.

• 论文 • 上一篇    

Establishing an antituberculosis model for screening traditional medicines

Peng Song, Yu Chen, Rui Zhang, Di Li, Wei Xu*   

  1. College of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016, China
  • 收稿日期:2015-05-01 修回日期:2015-07-12 出版日期:2015-08-20 发布日期:2015-08-20

Establishing an antituberculosis model for screening traditional medicines

Peng Song, Yu Chen, Rui Zhang, Di Li, Wei Xu*   

  1. College of Life Science and Biopharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2015-05-01 Revised:2015-07-12 Online:2015-08-20 Published:2015-08-20
  • Contact: Wei Xu

摘要： In this paper, a novobiocin super sensitive mutant Mycobacterium phlei S2 and a super resistant mutant M. phlei R2 were first obtained with the UV mutagenesis method. Based on the susceptibility differences among M. phlei 1180, M. phlei S2 and M. phlei R2, an antituberculosis primary screening model was established. Whether the target specificity (DNA gyrase subunit B) screening model was suitable for screening the drugs was investigated using the paper disk method. With the application of the screening model, three traditional medicine extracts were selected having obvious target specificity effect.

Abstract: In this paper, a novobiocin super sensitive mutant Mycobacterium phlei S2 and a super resistant mutant M. phlei R2 were first obtained with the UV mutagenesis method. Based on the susceptibility differences among M. phlei 1180, M. phlei S2 and M. phlei R2, an antituberculosis primary screening model was established. Whether the target specificity (DNA gyrase subunit B) screening model was suitable for screening the drugs was investigated using the paper disk method. With the application of the screening model, three traditional medicine extracts were selected having obvious target specificity effect.

Key words: Mycobacterium phlei, DNA gyrase, Screening model, Traditional medicines

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