Phytochemical analysis, antioxidant and hepatoprotective
activity of Roscoea purpurea, a Zingiberaceous herb
collected from Kumaun hills of Uttarakhand

亚洲传统医药

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Phytochemical analysis, antioxidant and hepatoprotective activity of Roscoea purpurea, a Zingiberaceous herb collected from Kumaun hills of Uttarakhand

Rahul Bathla 1, Sonali Sethi 1, Om Prakash 1*, H. Punetha 2, A.K. Pant 1, Munish Batra 3, Mahesh Kumar 4

1 Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar. U.S. Nagar, Uttarakhand, India; 2 Department of Biochemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar. U.S. Nagar, Uttarakhand, India; 3 Department of Veterinary Pathology, College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture & Technology, Pantnagar. U.S. Nagar, Uttarakhand, India; 4 Department of Veterinary Medicine, College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture & Technology, Pantnagar. U.S. Nagar, Uttarakhand, India

收稿日期:2014-05-30 修回日期:2016-07-06 出版日期:2016-12-20 发布日期:2016-07-06

Phytochemical analysis, antioxidant and hepatoprotective activity of Roscoea purpurea, a Zingiberaceous herb collected from Kumaun hills of Uttarakhand

Rahul Bathla 1, Sonali Sethi 1, Om Prakash 1*, H. Punetha 2, A.K. Pant 1, Munish Batra 3, Mahesh Kumar 4

1 Department of Chemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar. U.S. Nagar, Uttarakhand, India; 2 Department of Biochemistry, College of Basic Sciences and Humanities, G.B. Pant University of Agriculture & Technology, Pantnagar. U.S. Nagar, Uttarakhand, India; 3 Department of Veterinary Pathology, College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture & Technology, Pantnagar. U.S. Nagar, Uttarakhand, India; 4 Department of Veterinary Medicine, College of Veterinary and Animal Sciences, G.B. Pant University of Agriculture & Technology, Pantnagar. U.S. Nagar, Uttarakhand, India

Received:2014-05-30 Revised:2016-07-06 Online:2016-12-20 Published:2016-07-06
Contact: Om Prakasha, Department of Chemistry, College of Basic Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar 263 145, U.S. Nagar, Uttaerkhand, INDIA; Tel: +91-9411159551; E-mail: oporgchem@gmail.co, op_orgchem@yahoo.co.in.

摘要/Abstract

摘要： The present study aims for phytochemical analysis, hepatoprotective activity, antioxidant activity and total phenolic content of methanolic extract from rhizomes of R. purpurea. R. purpurea exhibited the presence of different type of phytochemicals like alkaloids, glycosides, phytosterol, phenol and flavonoids etc. The hepatoprotective activity in rats was studied by inducing oral dose at 250 mg/kg and 500 mg/kg b. wt., antioxidative activity was evaluated by different methods viz; the superoxide anion scavenging activity, hydroxyl radical scavenging activity, nitric oxide scavenging activity, DPPH radical scavenging activity, reducing power and total phenolic content. The rats were significantly reestablished from hepatotoxicity by analyzing the factors such as AST, ALT, glucose, triglycerides, cholesterol, total protein, bilirubin, albumin and urea. Further the histopathological results study revealed the significant improvement of liver. Methanolic extracts of rhizomes of R. purpurea showed abilities to scavenge hydroxyl, superoxide free radicals, nitric oxide, DPPH with IC50 values of 174.06, 63.14, 155.73, 119.85 μg/ml and also showed reducing power ability with the RP50 value of 280.41 μg/ml. The methanolic content was found to have a total phenolic content of 33.64 mg/g. The present study revealed that R. purpurea possess effective hepatoprotective and antioxidant potential and possibly might be used as herbal medicine to replace synthetic one.

关键词: Roscoea purpurea, hepatoprotective, antioxidant, total phenols, phytochemicals

Abstract: The present study aims for phytochemical analysis, hepatoprotective activity, antioxidant activity and total phenolic content of methanolic extract from rhizomes of R. purpurea. R. purpurea exhibited the presence of different type of phytochemicals like alkaloids, glycosides, phytosterol, phenol and flavonoids etc. The hepatoprotective activity in rats was studied by inducing oral dose at 250 mg/kg and 500 mg/kg b. wt., antioxidative activity was evaluated by different methods viz; the superoxide anion scavenging activity, hydroxyl radical scavenging activity, nitric oxide scavenging activity, DPPH radical scavenging activity, reducing power and total phenolic content. The rats were significantly reestablished from hepatotoxicity by analyzing the factors such as AST, ALT, glucose, triglycerides, cholesterol, total protein, bilirubin, albumin and urea. Further the histopathological results study revealed the significant improvement of liver. Methanolic extracts of rhizomes of R. purpurea showed abilities to scavenge hydroxyl, superoxide free radicals, nitric oxide, DPPH with IC50 values of 174.06, 63.14, 155.73, 119.85 μg/ml and also showed reducing power ability with the RP50 value of 280.41 μg/ml. The methanolic content was found to have a total phenolic content of 33.64 mg/g. The present study revealed that R. purpurea possess effective hepatoprotective and antioxidant potential and possibly might be used as herbal medicine to replace synthetic one.

Key words: Roscoea purpurea, hepatoprotective, antioxidant, total phenols, phytochemicals

Rahul Bathla,Sonali Sethi,Om Prakash,H.Punetha,AK.Pant,Munish Batra,Mahesh Kumar. Phytochemical analysis, antioxidant and hepatoprotective activity of Roscoea purpurea, a Zingiberaceous herb collected from Kumaun hills of Uttarakhand[J]. 亚洲传统医药.

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亚洲传统医药

Phytochemical analysis, antioxidant and hepatoprotective activity of Roscoea purpurea, a Zingiberaceous herb collected from Kumaun hills of Uttarakhand

Phytochemical analysis, antioxidant and hepatoprotective activity of Roscoea purpurea, a Zingiberaceous herb collected from Kumaun hills of Uttarakhand

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