亚洲传统医药

关键词: Neofusicoccum parvum, Elaeocarpus serratus, endophytic fungi, secondary metabolites, dihydroisocoumarines

Abstract: Neofusicoccum parvum, an endophytic fungus isolated from Elaeocarpus serratus (Ceylon Olive; family Elaeocarpaceae), was grown for 3 weeks in potato dextrose broth. Chromatographic separation of the ethyl acetate extracts from the culture filtrate and mycelium over silica gel, Sephadex LH-20 and preparative thin layer chromatography furnished (R)-7-hydroxymellein (1), (3R,4R)-4-hydroxymellein (2), (3R,4S)-4-hydroxymellein (3), (R)-5-hydroxymellein (4), (R)-mellein (5), (3R,4R)-4,7- dihydroxymellein (6), (6R,7S)-dia-asperlin (7), CJ-14445 (8) and 13,14,15,16-tetranorlabd-7-ene-19,6β:12,17-diolide (9). The structures of known compounds 1–9 were determined by spectroscopic methods and comparison with reported data. This is the first report of the isolation of an endophytic fungus from E. serratus, and the isolation of compounds 1, 4, 6, 8 and 9 from N. parvum. It is important to note that compounds 1–7 are small molecules with an oxygen heterocyclic ring system. These compounds can be used as starting materials in the synthesis of pharmaceutically important large molecules with oxygen heterocyles.

Key words: Neofusicoccum parvum, Elaeocarpus serratus, endophytic fungi, secondary metabolites, dihydroisocoumarines