亚洲传统医药

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2013年, 第8卷, 第4期　刊出日期：2013-08-20 上一期    下一期

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Chemical composition, antifungal and antioxidant activities of Perilla frutescens (L.) syn. P. ocimoides L. collected from different regions of Indian Himalaya Geetu Kunwar;Om Prakash;Mahesh Chandra;Anil Kumar Pant* 2013 (4):  88-98.  摘要 ( 409 )   Perilla (Lamiaceae) is cultivated mainly in Asian countries such as India, China, Korea, Vietnam, Thailand, and Japan. In present study six samples of Perilla frutescens (L.) syn. P. ocimoides L. were collected from Pantnagar, Almora, Birar, Bharsar, Ranibag, Chaurlekh of Uttarakhand in India. The essential oils analyzed by GC and GC-MS revealed the presence of several compounds in each essential oil of which the major constituents α-phellandrene, perillene, egomaketone, isoegomaketone, β-caryophyllene, germacrene-D, caryophyllene oxide were identified. Antifungal activity of essential oil of Perilla frutescens were tested against two pathogenic fungi viz. Rizoctonia solani Kuhn and Sclerotium rolfsii. All the oils exhibited antifungal activity but essential oil from Almora collection showed maximum activity against both the test fungi. All the essential oils and methanolic extracts of the plants were studied for their antioxidant activity by reducing power activity, chelating power and 2,2-diphenylpicrylhydrazyl (DPPH) radical scavenging activity which showed good to moderate antioxidant activity in a dose dependent manner which may be possibly due to different chemical and biochemical make up of the essential oils and extract. The phenolic contents in the methanolic extracts varied from 0.708- 4.750 mg/100 mg, flavonols from 0.040-0.195 mg/100 mg and ortho dihydric phenols from 0.175-0.705 mg/100 mg. 相关文章 | 计量指标

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In vitro phytochemical screening and antimicrobial activity of the leaves of Lawsonia inermis Safa H. AL-Aufi;Mohammad A. Hossain*;Afaf M. Weli;Qasim Al-Riyami 2013 (4):  99-105.  摘要 ( 342 )   The aim of this study is to investigate in vitro phytochemical analysis and antimicrobial activity of different solvents extracts from the fresh leaves of Omani Lawsonia inermis. Different polarities organic solvents such as methanol, chloroform, hexane, ethyl acetate and butanol were used to prepare the crude extracts from the fresh leaves of Lawsonia inermis. In vitro antimicrobial activity of different polarities extracts from the fresh leaves of Omani Lawsonia inermis was determined by agar disc diffusion method against one gram positive and two gram negative bacteria. Phytochemical screening of different polarities crude extracts were tested and shown positive results for alkaloids, flavonoids, glycosides, saponins, steroids, tannins and triterpenoids. However, the crude extracts didn’t show anthraquinones and phytosterol test. The methanol, chloroform, hexane, ethyl acetate and butanol crude extracts were display minimum antimicrobial potential against the tested one gram positive (Staphylococcus aureus) and two gram negative (Escherichia coli and Pseudomonus aeruginosa) bacterial strain at different concentrations in the range of 0-11%. Based on phytochemical screening and antimicrobial activity results it can be suggested that the fresh leaves extracts of Omani Lawsonia inermis for curing various formulations. 相关文章 | 计量指标

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Histological, pharmacognostic and preliminary phytochemical studies of Elytraria acaulis Anupama Singh;*;Vikas A. Saharan;Anil Bhandari 2013 (4):  106-118.  摘要 ( 537 )   Elytraria acaulis is traditionally used in treatment of burns, diarrhoea, throat compliments, fever, venereal diseases, kidney stone, tooth ache, asthma, migraine, leucorrhoea, piles, mammary tumours, pneumonia and infantile diarrhoea. The present study involves certain histological, pharmacognostical standardization and preliminary phytochemical investigations which may help in isolation, characterization of active constituents. Microscopy of different plant parts and powder of Elytraria acaulis were performed. Pharmacognostic standardization parameters were determined as per the WHO guidelines. Parameters like extractive value in different solvents, ash values and loss on drying were determined. Preliminary phytochemical studies, fluorescence analysis and chromatographic profiling were performed for the identification and characterisation of drug and its constituents. Starch and lignin are present while calcium oxalate was absent in all plant parts. In microscopic studies leaf constants were stomatal number 11.11±1.90, stomatal index 28.99±2.03, vein islet number 5.42±0.99, vein termination number 4.76±1.08, and palisade ratio 9 to 13. Chemoprofiling showed presence of alkaloids, tannins, flavonoids, steroids, volatile oils, and carbohydrates in both aerial and underground parts of plant, while proteins, glycosides and coumarins are present in underground parts while these are absent in aerial plant parts. The results of the performed studies are helpful in correct identification, characterisation of Elytraria acaulis. Preliminary phytochemical studies and chromatographic profiling may be helpful in further isolation and purification of lead compounds from different extracts. 相关文章 | 计量指标