亚洲传统医药

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2014年, 第9卷, 第3期　刊出日期：2014-06-20 上一期    下一期

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Protective effect of Wuling San on reproductivetoxicity induced by cisplatin in male mice Huili Weng;Jingyu Yang;Jingjing Zhang;Xiaoya Du;Shuang Gao;Chunfu Wu* 2014 (3):  52-61.  摘要 ( 483 )   Cisplatin (CP), an anticancer alkylating agent which can clinically cure testicular and ovarian cancer, has been reported to have serious toxic effects on the reproductive system. The aim of the present study was to investigate the possible protective effect of Wuling San (WLS) on CP-treated reproductive toxicity and compare the protective effect of WLS powder with that of a decoction. Male mice were given WLS powder (0.78, 2.34, 7.02 g/kg), WLS decoction or vitamin C (positive control) intragastrically twice a day for 14 consecutive days. CP was injected once a day, starting on day 9, for 5 consecutive days. The testes ratio, spermatic DNA damage, sperm DNA integrity, sperm count, sperm viability and a histopathological examination of the testis were carried out on day 14. A sperm abnormality test was carried out on day 35. The results obtained showed that WLS significantly reduced the rate of sperm DNA damage and the presence of abnormal sperm, increased the sperm count and sperm viability and the number of integral DNA sperm, and improved the testicular tissue morphology in the CP-exposed mice. In addition, there was no significant difference between the WLS powder and decoction on the protective effect against CP-treated reproductive toxicity. These results demonstrated that both WLS and its decoction had a protective effect against CP-treated reproductive toxicity in male mice and this will provide a reliable experimental basis for clinical drug treatment. 相关文章 | 计量指标

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An integrated metabonomic method fordiscovering biomarkers of Euphorbiae PekinensisRadix-induced hepatotoxicity and nephrotoxicity Pengyi Hou;Lulu Geng;Xu Zhao;Bingjie Ma;Yan Zeng;Zhenzhen Liu;Lu Li;Kaishun Bi;Xiaohui Chen * 2014 (3):  62-78.  摘要 ( 317 )   This paper was designed to investigate metabonomic characteristics of the hepatotoxicity and nephrotoxicity induced by Euphorbiae Pekinensis Radix (EPR), a commonly used traditional Chinese medicine which has been used for the treatment of edema, scrofula and antivirus. Metabonomic method was used for chronic toxicology study of EPR in rats. With a clinical dose, the rats of the model group were intragastricly administrated with the EPR extract for 10 weeks continuously. The serum samples of the model group and the healthy control group were collected at several time points and the endogenous metabolites were analyzed by UHPLC-MS/MS. Using the principal components analysis (PCA), the levels of thirteen endogenous metabolites were found to be significantly changed in serum from the model group. Metabonomics method could distinguish the model group from the healthy control group in 2nd, 6th and 10th week before the visible organic injuries of liver and kidney found in 10th week by histopathology approach. The present study proves the potential of UHPLC-MS/MS based metabonomics in mapping metabolic response for toxicology research. 相关文章 | 计量指标

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Activity of Brucea javanica oil injection againstgastric ulcer in rats Qian Li;Chen Liang;Qiujv Wang;Linlin Shi;Yan Qing;Jingjing Lv;Yuyang Zhang* 2014 (3):  79-87.  摘要 ( 461 )   This study is aimed at investigating the anti-gastric ulcer activity of Brucea javanica oil (BJO) on pylorus ligation (PL) and glacial acetic acid (GAA) -induced gastric ulcer in Wistar rats. BJO, the drug tested, was used to treat gastric ulcer and its potency observed at the doses of 0.15, 0.30 and 0.60 ml/kg (i.v.). Cimetidine was given intraperitoneally (i.p.) at a dose of 140 mg/kg as the positive control. Total acidity, inhibitory rate of gastric acid and activity of pepsin were measured in the rats with gastric ulcer induced by pylorus ligation. Ulcer scores as well as inhibitory rates of gastric ulcer were determined in the rats suffering pylorus ligation and those with glacial acetic acid injury respectively. In addition, hematoxylin and eosin (HE) staining for histopathologic examination was utilized to confirm further the effect of BJO against the gastric ulcer induced by pylorus ligation. The results show that BJO significantly reduces the ulcer scores, total acidity and the incidence of ulcer, and enhances the inhibitory effects on gastric ulcer and gastric acid production, although no significant changes were detected in the activity of pepsin. Based on the outcome of the treatment of the rats with BJO which was significantly superior to that of the control, it is inferred that Brucea javanica oil injection is an effective treatment for gastric ulcers in some of pathological models of rats. However, more investigation on precise mechanisms of the action is needed. 相关文章 | 计量指标