亚洲传统医药

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2014年, 第9卷, 第5期　刊出日期：2014-10-20 上一期    下一期

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Determination and pharmacokinetics of majorboswellic acids in rats plasma after oraladministration of an extract of Boswellia serrata Yunfeng Bai;Hongtao Jiang;Jinqi Wang;Mengjun Zhang;Xiaoyun Liu;Ronghua Dai* 2014 (5):  110-117.  摘要 ( 286 )   Boswellia serrata extract (BSE) has been used in traditional medicine for the treatment of inflammatory diseases since antiquity. 11- keto- β - boswellic acid (KBA) and 3- O- Acetyl- 11- keto- β- boswellic acid (AKBA) are the two major active ingredients in BSE. This study describes a simple and rapid HPLC method developed for quantification of KBA and AKBA in rat plasma after administration of BSE. Separation was performed on a YMC-Pack ODS-A column (150 mm × 4.6 mm, 5 μm) using a mobile phase composed of acetonitrile and aqueous 0.5% phosphoric acid pumped at a flow rate of 1.0 ml/min with gradient elution. Detection was performed at a wavelength of 250 nm and the operating temperature was maintained at 35°C. The lower limits of quantification were 0.1296 μg/ml, 0.1373 μg/ml for KBA and AKBA , respectively, and the intra - and inter-day precision and accuracy of analytes were well within acceptance criteria (15%). The mean extraction recoveries of the analytes and IS from rat plasma were all more than 85.0%. The validated method was successfully applied to determine the pharmacokinetic profiles of the analytes in rat plasma. 相关文章 | 计量指标

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Pharmacognostical studies on Ehretia microphylla Lamk Aarthi V, Shakila R , Sasikala E, Pitchiahkumar M 2014 (5):  118-129.  摘要 ( 379 )   Ehretia microphylla Lamk. is a member of the Boraginaceae family and finds its use in the Siddha System of Medicine. Aim of this study is to investigate the morphology, exo and endomorphic characters, powder analysis, preliminary phytochemical analysis, pharmacopoeial standards such as physico-chemical parameter, TLC photo documentation and HPTLC finger print of aerial parts. The results revealed the presence of an arc shaped collateral vascular bundles in the centre of the midrib and discontinuous sclerenchyma fibres below the vascular bundle. The adaxial and abaxial epidermii are nearly identical in structure but differ in their sizes. Presence of aerenchymatous spongy tissue, tanniniferous sacs and cluster crystals of calcium oxalate in the mesophyll and 2 or 3 layered hypodermis and isolated strands of pericyclic fibres in the stem are characteristic features. The powder showed abundant unicellular trichomes, foliar epidermal cells with anomocytic (ranunculaceous) stomata and druses of calcium oxalate crystals which are diagnostic characters to identify the drug. HPTLC finger printing showed 16 peaks at 254 nm; the TLC photo documentation showed four visible spots under 254 nm, 8 spots under 366 nm and 4 spots after derivatization. 相关文章 | 计量指标

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Molecular identification based on ITS sequencesfor Panax ginseng and its adulterants Yu Chen;Wei Xu;Peng Song;Xing Wei;Shukun Ma;Shiyi Xu * 2014 (5):  130-135.  摘要 ( 318 )   Panax ginseng has been traditionally regarded as having medicinal properties. However, reports are rare about the molecular organization and sequence variation in the nuclear ribosomal DNA (rDNA) region, including the two internal transcribed spacers (ITS1 and ITS2) between P. ginseng and its adulterants. The aim of this study is to evaluate the differences between P. ginseng and its adulterants. Genomic DNA extracted from P. ginseng and its two adulterants were used as templates. The ITS regions were amplified using a polymerase chain reaction. Sequence assembly was performed using DNAMAN Version 6.0.3.99 and MEGA 5.0 software. The results obtained indicate that the ITS sequences could be used to identify P. ginseng and distinguish it from its two adulterants. 相关文章 | 计量指标